gamma irradiated dr4 mef feeder cells Search Results


mouse monoclonal antibodies anti trail receptors 1 4  (R&D Systems)
93
R&D Systems mouse monoclonal antibodies anti trail receptors 1 4
Mouse Monoclonal Antibodies Anti Trail Receptors 1 4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+irradiated+dr4+mef+feeder+cells/Negative+Control+for+Mouse+Monoclonal+Antibody+(IGG1%2F453)/pmc06013189-179-0-8
Average 93 stars, based on 1 article reviews
mouse monoclonal antibodies anti trail receptors 1 4 - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
gamma irradiated drug resistant dr4 irmef feeder cells  (ATCC)
96
ATCC gamma irradiated drug resistant dr4 irmef feeder cells
Gamma Irradiated Drug Resistant Dr4 Irmef Feeder Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+irradiated+dr4+mef+feeder+cells/MEF/bio_rxiv__2025__07__16__665154-199-28-34
Average 96 stars, based on 1 article reviews
gamma irradiated drug resistant dr4 irmef feeder cells - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier
goat antihuman dr4 igg  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology goat antihuman dr4 igg
Figure 1 Immunohistochemical analysis of death receptors <t>DR4</t> (A, C, and E) and DR5 (B, D, and F) from tissue microarray of GCTs. Examples of strong, intermediate, and low expression are depicted in upper, middle, and lower rows respectively. Higher magnifications are shown in insets. Brown color indicates positive staining. Scale bar is 50 mm.
Goat Antihuman Dr4 Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+irradiated+dr4+mef+feeder+cells/goat+anti-mouse+IgG-B/10__1677_slash_erc___10___0041-61-47-55
Average 96 stars, based on 1 article reviews
goat antihuman dr4 igg - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier
trail r1 dr4  (OriGene)
90
OriGene trail r1 dr4
Figure 3. High expression of CD95 and <t>TRAIL</t> receptors in CTCL cells. Surface expression of death receptors <t>(DR4,</t> DR5, and CD95) was determined by flow cytometry for the CTCL and T-ALL cell lines. The cells were stained with (a) specific monoclonal TRAIL-R1/DR4, (b) TRAIL-R2/DR5, and (c) CD95-specific antibody (filled graphs). Isotype control antibodies were used as negative controls (open graphs). A shift to the right indicates increased surface expression. (d) Median expression values obtained for the individual cell lines (each two independent experiments) were normalized with respect to the mean expression in all eight cell lines. (+) indicates the loss of CD95 expression in SeAx.
Trail R1 Dr4, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+irradiated+dr4+mef+feeder+cells/DR4+(TNFRSF10A)+Rabbit+Polyclonal+Antibody/pm17495957-201-5-11
Average 90 stars, based on 1 article reviews
trail r1 dr4 - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
murine igg monoclonal anti dr4 antibody  (Novus Biologicals)
96
Novus Biologicals murine igg monoclonal anti dr4 antibody
Figure 3. High expression of CD95 and <t>TRAIL</t> receptors in CTCL cells. Surface expression of death receptors <t>(DR4,</t> DR5, and CD95) was determined by flow cytometry for the CTCL and T-ALL cell lines. The cells were stained with (a) specific monoclonal TRAIL-R1/DR4, (b) TRAIL-R2/DR5, and (c) CD95-specific antibody (filled graphs). Isotype control antibodies were used as negative controls (open graphs). A shift to the right indicates increased surface expression. (d) Median expression values obtained for the individual cell lines (each two independent experiments) were normalized with respect to the mean expression in all eight cell lines. (+) indicates the loss of CD95 expression in SeAx.
Murine Igg Monoclonal Anti Dr4 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+irradiated+dr4+mef+feeder+cells/Negative+Control+for+Mouse+Monoclonal+Antibody+(IGG1%2F453)/pm18245494-80-6-11
Average 96 stars, based on 1 article reviews
murine igg monoclonal anti dr4 antibody - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier
igg1 isotype control antibodies  (R&D Systems)
96
R&D Systems igg1 isotype control antibodies
Figure 3. High expression of CD95 and <t>TRAIL</t> receptors in CTCL cells. Surface expression of death receptors <t>(DR4,</t> DR5, and CD95) was determined by flow cytometry for the CTCL and T-ALL cell lines. The cells were stained with (a) specific monoclonal TRAIL-R1/DR4, (b) TRAIL-R2/DR5, and (c) CD95-specific antibody (filled graphs). Isotype control antibodies were used as negative controls (open graphs). A shift to the right indicates increased surface expression. (d) Median expression values obtained for the individual cell lines (each two independent experiments) were normalized with respect to the mean expression in all eight cell lines. (+) indicates the loss of CD95 expression in SeAx.
Igg1 Isotype Control Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+irradiated+dr4+mef+feeder+cells/Mouse+IgG1+Isotype+Control/pm17309775-88-10-37
Average 96 stars, based on 1 article reviews
igg1 isotype control antibodies - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier
allophycocyanin apc conjugated trail r1  (R&D Systems)
94
R&D Systems allophycocyanin apc conjugated trail r1
<t>TRAIL</t> sensitivity by in vitro characterization. ( A ) The half maximal inhibitory concentration (IC 50 ) value was determined in non-small cell lung cancer (NSCLC) cell lines (H460, H2170 and A549) by MTS assay after 48 h of rhTRAIL treatment at different concentrations. ( B ) Analysis of <t>TRAIL</t> <t>receptors</t> expression [agonist receptors <t>(DR4</t> and DR5) and decoy receptors (DcR1 and DcR2)] in MSCs and NSCLC cell lines by flow cytometry. ( C ) Sensitivity of NSCLC cell lines and MSCs evaluated by MTS assay after rhTRAIL (500 ng/mL) treatment.
Allophycocyanin Apc Conjugated Trail R1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+irradiated+dr4+mef+feeder+cells/Human+TRAILR1%2FTNFRSF10A+APC-conjugated+Antibody/pmc06770521-202-20-53
Average 94 stars, based on 1 article reviews
allophycocyanin apc conjugated trail r1 - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier
monoclonal antibodies against apo2l/trail-r1/dr4  (Immunex Corporation)
90
Immunex Corporation monoclonal antibodies against apo2l/trail-r1/dr4
<t>TRAIL</t> sensitivity by in vitro characterization. ( A ) The half maximal inhibitory concentration (IC 50 ) value was determined in non-small cell lung cancer (NSCLC) cell lines (H460, H2170 and A549) by MTS assay after 48 h of rhTRAIL treatment at different concentrations. ( B ) Analysis of <t>TRAIL</t> <t>receptors</t> expression [agonist receptors <t>(DR4</t> and DR5) and decoy receptors (DcR1 and DcR2)] in MSCs and NSCLC cell lines by flow cytometry. ( C ) Sensitivity of NSCLC cell lines and MSCs evaluated by MTS assay after rhTRAIL (500 ng/mL) treatment.
Monoclonal Antibodies Against Apo2l/Trail R1/Dr4, supplied by Immunex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+irradiated+dr4+mef+feeder+cells/recombinant+human+trail/pm16550602-48-10-30
Average 90 stars, based on 1 article reviews
monoclonal antibodies against apo2l/trail-r1/dr4 - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
phycoerythrin pe conjugated goat anti mouse igg  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology phycoerythrin pe conjugated goat anti mouse igg
<t>TRAIL</t> sensitivity by in vitro characterization. ( A ) The half maximal inhibitory concentration (IC 50 ) value was determined in non-small cell lung cancer (NSCLC) cell lines (H460, H2170 and A549) by MTS assay after 48 h of rhTRAIL treatment at different concentrations. ( B ) Analysis of <t>TRAIL</t> <t>receptors</t> expression [agonist receptors <t>(DR4</t> and DR5) and decoy receptors (DcR1 and DcR2)] in MSCs and NSCLC cell lines by flow cytometry. ( C ) Sensitivity of NSCLC cell lines and MSCs evaluated by MTS assay after rhTRAIL (500 ng/mL) treatment.
Phycoerythrin Pe Conjugated Goat Anti Mouse Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+irradiated+dr4+mef+feeder+cells/goat+anti-mouse+IgG-PE/10__5352_slash_jls__2016__26__4__431-51-24-29
Average 96 stars, based on 1 article reviews
phycoerythrin pe conjugated goat anti mouse igg - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier
goat polyclonal igg for dr4  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology goat polyclonal igg for dr4
<t>TRAIL</t> sensitivity by in vitro characterization. ( A ) The half maximal inhibitory concentration (IC 50 ) value was determined in non-small cell lung cancer (NSCLC) cell lines (H460, H2170 and A549) by MTS assay after 48 h of rhTRAIL treatment at different concentrations. ( B ) Analysis of <t>TRAIL</t> <t>receptors</t> expression [agonist receptors <t>(DR4</t> and DR5) and decoy receptors (DcR1 and DcR2)] in MSCs and NSCLC cell lines by flow cytometry. ( C ) Sensitivity of NSCLC cell lines and MSCs evaluated by MTS assay after rhTRAIL (500 ng/mL) treatment.
Goat Polyclonal Igg For Dr4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+irradiated+dr4+mef+feeder+cells/mouse+anti-goat+IgG-FITC/10__1200_slash_jco__2006__06__8809-60-35-43
Average 95 stars, based on 1 article reviews
goat polyclonal igg for dr4 - by Bioz Stars, 2026-11
95/100 stars
  Buy from Supplier
horseradish peroxidase conjugated goat anti rabbit igg  (Bio-Rad)
96
Bio-Rad horseradish peroxidase conjugated goat anti rabbit igg
<t>TRAIL</t> sensitivity by in vitro characterization. ( A ) The half maximal inhibitory concentration (IC 50 ) value was determined in non-small cell lung cancer (NSCLC) cell lines (H460, H2170 and A549) by MTS assay after 48 h of rhTRAIL treatment at different concentrations. ( B ) Analysis of <t>TRAIL</t> <t>receptors</t> expression [agonist receptors <t>(DR4</t> and DR5) and decoy receptors (DcR1 and DcR2)] in MSCs and NSCLC cell lines by flow cytometry. ( C ) Sensitivity of NSCLC cell lines and MSCs evaluated by MTS assay after rhTRAIL (500 ng/mL) treatment.
Horseradish Peroxidase Conjugated Goat Anti Rabbit Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+irradiated+dr4+mef+feeder+cells/Goat+anti+Rabbit+IgG/pm11163110-84-11-21
Average 96 stars, based on 1 article reviews
horseradish peroxidase conjugated goat anti rabbit igg - by Bioz Stars, 2026-11
96/100 stars
  Buy from Supplier
mouse monoclonal antibodies against human trail receptor type 4 igg2a hutrail r4-m445  (Immunex Corporation)
90
Immunex Corporation mouse monoclonal antibodies against human trail receptor type 4 igg2a hutrail r4-m445
<t>TRAIL</t> sensitivity by in vitro characterization. ( A ) The half maximal inhibitory concentration (IC 50 ) value was determined in non-small cell lung cancer (NSCLC) cell lines (H460, H2170 and A549) by MTS assay after 48 h of rhTRAIL treatment at different concentrations. ( B ) Analysis of <t>TRAIL</t> <t>receptors</t> expression [agonist receptors <t>(DR4</t> and DR5) and decoy receptors (DcR1 and DcR2)] in MSCs and NSCLC cell lines by flow cytometry. ( C ) Sensitivity of NSCLC cell lines and MSCs evaluated by MTS assay after rhTRAIL (500 ng/mL) treatment.
Mouse Monoclonal Antibodies Against Human Trail Receptor Type 4 Igg2a Hutrail R4 M445, supplied by Immunex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gamma+irradiated+dr4+mef+feeder+cells/igg2a+hutrailm271/pm15798435-58-26-36
Average 90 stars, based on 1 article reviews
mouse monoclonal antibodies against human trail receptor type 4 igg2a hutrail r4-m445 - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

Image Search Results


Figure 1 Immunohistochemical analysis of death receptors DR4 (A, C, and E) and DR5 (B, D, and F) from tissue microarray of GCTs. Examples of strong, intermediate, and low expression are depicted in upper, middle, and lower rows respectively. Higher magnifications are shown in insets. Brown color indicates positive staining. Scale bar is 50 mm.

Journal: Endocrine-Related Cancer

Article Title: GATA4 protects granulosa cell tumors from TRAIL-induced apoptosis

doi: 10.1677/erc-10-0041

Figure Lengend Snippet: Figure 1 Immunohistochemical analysis of death receptors DR4 (A, C, and E) and DR5 (B, D, and F) from tissue microarray of GCTs. Examples of strong, intermediate, and low expression are depicted in upper, middle, and lower rows respectively. Higher magnifications are shown in insets. Brown color indicates positive staining. Scale bar is 50 mm.

Article Snippet: Primary antibodies were as follows: mouse antihuman a-inhibin IgG (MCA 9515, AbD Serotec, Oxford, UK) at dilution of 1:100, goat antihuman antiMüllerian hormone (AMH) IgG (sc-6886, Santa Cruz Biotechnology, Inc.) at dilution of 1:100, and goat antihuman GATA4 IgG (sc-1237, Santa Cruz Biotechnology) at dilution of 1:200; goat antihuman DR4 IgG at 1:100 dilution (sc-7863, Santa Cruz Biotechnology), goat antihuman DR5 IgG at 1:50 dilution (sc-7192, Santa Cruz Biotechnology), rabbit antihuman DR4 IgG at 1:400 dilution (ab8414, Abcam, Cambridge, UK), and DR5 IgG at 1:400 dilution (ab8416, Abcam).

Techniques: Immunohistochemical staining, Microarray, Expressing, Staining

Figure 2 Immunohistochemical analysis of TRAIL receptors DR4 (A), DR5 (B), and GCT markers AMH (C), a-inhibin (inh-a) (D), and GATA4 (E) in cultured GCT cells. Brown color indicates positive staining. (F) A negative control using secondary antibody only. Higher magnifications are shown in insets. Scale bar is 50 mm.

Journal: Endocrine-Related Cancer

Article Title: GATA4 protects granulosa cell tumors from TRAIL-induced apoptosis

doi: 10.1677/erc-10-0041

Figure Lengend Snippet: Figure 2 Immunohistochemical analysis of TRAIL receptors DR4 (A), DR5 (B), and GCT markers AMH (C), a-inhibin (inh-a) (D), and GATA4 (E) in cultured GCT cells. Brown color indicates positive staining. (F) A negative control using secondary antibody only. Higher magnifications are shown in insets. Scale bar is 50 mm.

Article Snippet: Primary antibodies were as follows: mouse antihuman a-inhibin IgG (MCA 9515, AbD Serotec, Oxford, UK) at dilution of 1:100, goat antihuman antiMüllerian hormone (AMH) IgG (sc-6886, Santa Cruz Biotechnology, Inc.) at dilution of 1:100, and goat antihuman GATA4 IgG (sc-1237, Santa Cruz Biotechnology) at dilution of 1:200; goat antihuman DR4 IgG at 1:100 dilution (sc-7863, Santa Cruz Biotechnology), goat antihuman DR5 IgG at 1:50 dilution (sc-7192, Santa Cruz Biotechnology), rabbit antihuman DR4 IgG at 1:400 dilution (ab8414, Abcam, Cambridge, UK), and DR5 IgG at 1:400 dilution (ab8416, Abcam).

Techniques: Immunohistochemical staining, Cell Culture, Staining, Negative Control

Figure 3. High expression of CD95 and TRAIL receptors in CTCL cells. Surface expression of death receptors (DR4, DR5, and CD95) was determined by flow cytometry for the CTCL and T-ALL cell lines. The cells were stained with (a) specific monoclonal TRAIL-R1/DR4, (b) TRAIL-R2/DR5, and (c) CD95-specific antibody (filled graphs). Isotype control antibodies were used as negative controls (open graphs). A shift to the right indicates increased surface expression. (d) Median expression values obtained for the individual cell lines (each two independent experiments) were normalized with respect to the mean expression in all eight cell lines. (+) indicates the loss of CD95 expression in SeAx.

Journal: The Journal of investigative dermatology

Article Title: Blockade of death receptor-mediated pathways early in the signaling cascade coincides with distinct apoptosis resistance in cutaneous T-cell lymphoma cells.

doi: 10.1038/sj.jid.5700868

Figure Lengend Snippet: Figure 3. High expression of CD95 and TRAIL receptors in CTCL cells. Surface expression of death receptors (DR4, DR5, and CD95) was determined by flow cytometry for the CTCL and T-ALL cell lines. The cells were stained with (a) specific monoclonal TRAIL-R1/DR4, (b) TRAIL-R2/DR5, and (c) CD95-specific antibody (filled graphs). Isotype control antibodies were used as negative controls (open graphs). A shift to the right indicates increased surface expression. (d) Median expression values obtained for the individual cell lines (each two independent experiments) were normalized with respect to the mean expression in all eight cell lines. (+) indicates the loss of CD95 expression in SeAx.

Article Snippet: The following antibodies were used: TRAIL-R1/ DR4 (rabbit polyclonal IgG, ab8414; Acris Antibodies, Hiddenhau- sen, Germany, 1:500); TRAIL-R2/DR5 (rabbit polyclonal IgG, ab8416, Acris Antibodies, 1:500); TNF-R1 (mouse monoclonal IgG, H5, sc-8436; Santa Cruz, Heidelberg, Germany, 1:200); caspase-8 (mouse monoclonal IgG, 1C12; Cell Signaling, Danvers, 1:1,000); caspase-10 (mouse monoclonal IgG, 4C1; MBL Interna- 2434 Journal of Investigative Dermatology (2007), Volume 127 tional, Watertown, MA, 1:1,000); caspase-9 (rabbit polyclonal IgG; Cell Signaling, 1:1,000); caspase-3 cleavage products (rabbit polyclonal IgG, Asp175; Cell Signaling, 1:1,000); procaspase-3 (rabbit polyclonal IgG; Cell Signaling, 1:1,000); FADD (rabbit polyclonal IgG; Cell Signaling, 1:1,000); c-FLIP (mouse monoclonal IgG, G-11, sc-5276, Santa Cruz, 1:200); Bid (rabbit, polyclonal IgG; Cell Signaling, 1:1,000); Bax (rabbit polyclonal IgG, N-20, sc- 493; Santa Cruz, 1:200); Bcl-2 (mouse monoclonal IgG, sc-509; Santa Cruz, 1:200); Bcl-xL (rabbit polyclonal IgG, S-18, sc-634; Santa Cruz, 1:200); survivin (mouse monoclonal IgG, D-8, sc-17779; Santa Cruz, 1:200); X-linked inhibitor of apoptosis (rabbit polyclonal IgG; Cell Signaling, 1:1,000); a-tubulin (rabbit polyclonal IgG; Cell Signaling, 1:1,000); glyceraldehyde-3-phosphate dehydro- genase (mouse monoclonal IgG, 6C5, sc-32233; Santa Cruz, 1:200); b-actin (mouse monoclonal IgG, A5541; Sigma-Aldrich, Seelze, Germany, 1:5,000).

Techniques: Expressing, Flow Cytometry, Staining, Control

Figure 5. Weak expression of TNF-R1, caspase-10, and Bid in CTCL cell lines. Western blot analysis for (a) DR4, DR5, and TNF-R1, (b) for procaspase-3, -8, and -10 as well as for (c) FADD, Bid, and caspase-9 are shown for CTCL cell lines (HH, HuT-78, MyLa, SeAx) and for T-ALL cell lines (Jurkat, CCRF-CEM, MOLT-4, Karpas-45). Molecular weights are indicated as 44 (DR4), 47/40 (DR5), 55 (TNF-R1), 30 (caspase-3), 55/53 (caspase-8), 60/55 (caspase-10), 25 (FADD), 18 (Bid), 45 (caspase-9), 50 (a-tubulin), and 37 kDa (glyceraldehyde-3-phosphate dehydrogenase). Identical protein amounts (25 mg per lane) had been loaded to each lane. Consistent blotting was confirmed by Ponceau red staining and evaluation of a-tubulin or GAPDH expression. Expression of Bid in MyLa cells was not detectable here as short exposure times were used for chemiluminescence detection to avoid signal saturation in other cell lines. However, weak expression of Bid can be seen in MyLa after longer exposure, as shown in Figure 2, whereas HH remained negative. Two experiments performed for each cell line starting from independent cultures revealed reproducible results.

Journal: The Journal of investigative dermatology

Article Title: Blockade of death receptor-mediated pathways early in the signaling cascade coincides with distinct apoptosis resistance in cutaneous T-cell lymphoma cells.

doi: 10.1038/sj.jid.5700868

Figure Lengend Snippet: Figure 5. Weak expression of TNF-R1, caspase-10, and Bid in CTCL cell lines. Western blot analysis for (a) DR4, DR5, and TNF-R1, (b) for procaspase-3, -8, and -10 as well as for (c) FADD, Bid, and caspase-9 are shown for CTCL cell lines (HH, HuT-78, MyLa, SeAx) and for T-ALL cell lines (Jurkat, CCRF-CEM, MOLT-4, Karpas-45). Molecular weights are indicated as 44 (DR4), 47/40 (DR5), 55 (TNF-R1), 30 (caspase-3), 55/53 (caspase-8), 60/55 (caspase-10), 25 (FADD), 18 (Bid), 45 (caspase-9), 50 (a-tubulin), and 37 kDa (glyceraldehyde-3-phosphate dehydrogenase). Identical protein amounts (25 mg per lane) had been loaded to each lane. Consistent blotting was confirmed by Ponceau red staining and evaluation of a-tubulin or GAPDH expression. Expression of Bid in MyLa cells was not detectable here as short exposure times were used for chemiluminescence detection to avoid signal saturation in other cell lines. However, weak expression of Bid can be seen in MyLa after longer exposure, as shown in Figure 2, whereas HH remained negative. Two experiments performed for each cell line starting from independent cultures revealed reproducible results.

Article Snippet: The following antibodies were used: TRAIL-R1/ DR4 (rabbit polyclonal IgG, ab8414; Acris Antibodies, Hiddenhau- sen, Germany, 1:500); TRAIL-R2/DR5 (rabbit polyclonal IgG, ab8416, Acris Antibodies, 1:500); TNF-R1 (mouse monoclonal IgG, H5, sc-8436; Santa Cruz, Heidelberg, Germany, 1:200); caspase-8 (mouse monoclonal IgG, 1C12; Cell Signaling, Danvers, 1:1,000); caspase-10 (mouse monoclonal IgG, 4C1; MBL Interna- 2434 Journal of Investigative Dermatology (2007), Volume 127 tional, Watertown, MA, 1:1,000); caspase-9 (rabbit polyclonal IgG; Cell Signaling, 1:1,000); caspase-3 cleavage products (rabbit polyclonal IgG, Asp175; Cell Signaling, 1:1,000); procaspase-3 (rabbit polyclonal IgG; Cell Signaling, 1:1,000); FADD (rabbit polyclonal IgG; Cell Signaling, 1:1,000); c-FLIP (mouse monoclonal IgG, G-11, sc-5276, Santa Cruz, 1:200); Bid (rabbit, polyclonal IgG; Cell Signaling, 1:1,000); Bax (rabbit polyclonal IgG, N-20, sc- 493; Santa Cruz, 1:200); Bcl-2 (mouse monoclonal IgG, sc-509; Santa Cruz, 1:200); Bcl-xL (rabbit polyclonal IgG, S-18, sc-634; Santa Cruz, 1:200); survivin (mouse monoclonal IgG, D-8, sc-17779; Santa Cruz, 1:200); X-linked inhibitor of apoptosis (rabbit polyclonal IgG; Cell Signaling, 1:1,000); a-tubulin (rabbit polyclonal IgG; Cell Signaling, 1:1,000); glyceraldehyde-3-phosphate dehydro- genase (mouse monoclonal IgG, 6C5, sc-32233; Santa Cruz, 1:200); b-actin (mouse monoclonal IgG, A5541; Sigma-Aldrich, Seelze, Germany, 1:5,000).

Techniques: Expressing, Western Blot, Staining

TRAIL sensitivity by in vitro characterization. ( A ) The half maximal inhibitory concentration (IC 50 ) value was determined in non-small cell lung cancer (NSCLC) cell lines (H460, H2170 and A549) by MTS assay after 48 h of rhTRAIL treatment at different concentrations. ( B ) Analysis of TRAIL receptors expression [agonist receptors (DR4 and DR5) and decoy receptors (DcR1 and DcR2)] in MSCs and NSCLC cell lines by flow cytometry. ( C ) Sensitivity of NSCLC cell lines and MSCs evaluated by MTS assay after rhTRAIL (500 ng/mL) treatment.

Journal: Cancers

Article Title: Targeting of CD133+ Cancer Stem Cells by Mesenchymal Stem Cell Expressing TRAIL Reveals a Prospective Role of Apoptotic Gene Regulation in Non-Small Cell Lung Cancer

doi: 10.3390/cancers11091261

Figure Lengend Snippet: TRAIL sensitivity by in vitro characterization. ( A ) The half maximal inhibitory concentration (IC 50 ) value was determined in non-small cell lung cancer (NSCLC) cell lines (H460, H2170 and A549) by MTS assay after 48 h of rhTRAIL treatment at different concentrations. ( B ) Analysis of TRAIL receptors expression [agonist receptors (DR4 and DR5) and decoy receptors (DcR1 and DcR2)] in MSCs and NSCLC cell lines by flow cytometry. ( C ) Sensitivity of NSCLC cell lines and MSCs evaluated by MTS assay after rhTRAIL (500 ng/mL) treatment.

Article Snippet: The NSCLC cell lines were analyzed for TRAIL agonist (DR4 and DR5) and other decoy receptors (DcR1 and DcR2) with allophycocyanin (APC)-conjugated TRAIL-R1 (DR4) (cat no: FAB347A; Mouse IgG1), TRAIL-R2 (DR5) (cat no: FAB6311A; Mouse IgG 2B ), TRAIL-R3 (DcR1) (cat no: FAB6302A; Mouse IgG1), and TRAIL-R4 (DcR2) (cat no: FAB633A; Mouse IgG1) (R&D Systems, Minneapolis, MN, USA) using flow cytometry (Becton Dickinson BD).

Techniques: In Vitro, Concentration Assay, MTS Assay, Expressing, Flow Cytometry

Flow cytometry analysis of CD133+ NSCLC-derived cancer stem cells (CSCs) and their TRAIL receptor expression. ( A ) Cell debris and doublets were discriminated, and dead cells were excluded [(DAPI/4′,6-diamidino-2-phenylindole) negative; shown by the first four panels], followed by isolation of the CD133+ population in NSCLC cell lines (A549, H2170, and H460) in the lower panels. ( B ) Both CD133+ CSCs of H2170 and H460 expressed a high level of TRAIL cognate receptor (DR5), consistent with their sensitivity to TRAIL.

Journal: Cancers

Article Title: Targeting of CD133+ Cancer Stem Cells by Mesenchymal Stem Cell Expressing TRAIL Reveals a Prospective Role of Apoptotic Gene Regulation in Non-Small Cell Lung Cancer

doi: 10.3390/cancers11091261

Figure Lengend Snippet: Flow cytometry analysis of CD133+ NSCLC-derived cancer stem cells (CSCs) and their TRAIL receptor expression. ( A ) Cell debris and doublets were discriminated, and dead cells were excluded [(DAPI/4′,6-diamidino-2-phenylindole) negative; shown by the first four panels], followed by isolation of the CD133+ population in NSCLC cell lines (A549, H2170, and H460) in the lower panels. ( B ) Both CD133+ CSCs of H2170 and H460 expressed a high level of TRAIL cognate receptor (DR5), consistent with their sensitivity to TRAIL.

Article Snippet: The NSCLC cell lines were analyzed for TRAIL agonist (DR4 and DR5) and other decoy receptors (DcR1 and DcR2) with allophycocyanin (APC)-conjugated TRAIL-R1 (DR4) (cat no: FAB347A; Mouse IgG1), TRAIL-R2 (DR5) (cat no: FAB6311A; Mouse IgG 2B ), TRAIL-R3 (DcR1) (cat no: FAB6302A; Mouse IgG1), and TRAIL-R4 (DcR2) (cat no: FAB633A; Mouse IgG1) (R&D Systems, Minneapolis, MN, USA) using flow cytometry (Becton Dickinson BD).

Techniques: Flow Cytometry, Derivative Assay, Expressing, Isolation